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KMID : 1098420050130030109
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 3 p.109 ~ p.113
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Genetic Relationship among Ostericum koreanum Kitakawa Collections by RAPD Analysis
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Kim Soo-Young
Sim Yong-Gu
Kwon Soon-Tae
Oh Sei-Myung
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Abstract
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To analyze the genetic relationship 8 accessions of Ostericum koreanum Kitakawa, random amplified polymorphic DNA(RAPD) analysis was performed using 60 Operon primers. The 25 primers out of 60 random primers were amplified DNA by PCR using genomic DNA of O. koreanum. Eighty-five (49.1%) among 173 bands derived from 25 primers showed poly morphism, On the basis of similarity coefficient analysis by UPGMA, 8 accessions of O. koreanum Kitakawa could be classified into three groups at the similarity coefficient value of 0.71. Group I contained three accessions (Nam Gangwhal), Group II contained one accession (Nam Gangwhal) and Group III contained four accessions (Buk Gangwhal), The range of total genetic similarity coefficient value of 8 accessions of O. koreanum Kitakawa was 0.63{sim}0.96. Buk Gangwhal was flowered 18 to 26 days earlier than Nam Gangwhal, and Nam Gangwhal leaf stalk was thin and long as bolting rate high compared with Buk Gangwhal.
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KEYWORD
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Ostcricum koreanum, RAPD, genetic relationship
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